Article
Structural effects induced by removal of a disulfide-bridge: the X-ray structure of the C30A/C51A mutant of basic pancreatic trypsin inhibitor at 1.6 A.
Protein engineering - 1 Jul 1990
Eigenbrot C, Randal M, Kossiakoff A A
Abstract excerpt
The X-ray structure of a variant of basic pancreatic trypsin inhibitor (BPTI) has been analyzed to determine the structural accommodation resulting from removal of a disulfide cross-link in a protein. The disulfide removed, Cys30-Cys51, has been implicated in both the folding pathway of the protein and its overall thermal stability. In the variant studied, C30A/C51A, the disulfide cysteines were replaced by less...
Topics
- Aprotinin
- Disulfides
- Escherichia coli
- Fourier Analysis
- Models, Molecular
- Mutation
- Protein Conformation
- Water
- X-Ray Diffraction