Transport properties of Asp-51-->Glu and Asp-120-->Glu mutants of the melibiose carrier of Escherichia coli.

doi:10.1016/0005-2736(94)90078-7

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Article

Transport properties of Asp-51-->Glu and Asp-120-->Glu mutants of the melibiose carrier of Escherichia coli.

Biochimica et biophysica acta - 23 Mar 1994

Wilson D M, Wilson T H

Abstract excerpt

Asp-51-->Glu and Asp-120-->Glu mutants of the melibiose carrier of Escherichia coli were investigated for their cation/sugar cotransport properties. The carrier containing Glu-51 showed proton/melibiose cotransport but was extremely defective in Na+ or Li+ stimulation of sugar accumulation. On th...

Topics

Biological Transport
Carrier Proteins
Escherichia coli
Lithium
Melibiose
Mutagenesis, Site-Directed
Mutation
Sodium

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