Article
Aspartate 55 in the Na+/proline permease of Escherichia coli is essential for Na+-coupled proline uptake.
Biochemistry - 15 Apr 1997
Quick M, Jung H
Abstract excerpt
Four acidic residues in the N-terminal domain of Na+/proline permease of Escherichia coli (Asp33, Asp34, and Asp55 in putative loop 2, Glu75 in putative transmembrane domain II) were individually replaced with neutral or charged amino acid residues. Replacement of Glu75, the only residue in the p...
Topics
- Amino Acid Sequence
- Amino Acid Transport Systems, Neutral
- Amino Acids
- Aspartic Acid
- Biological Transport, Active
- Blotting, Western
- Diffusion
- Dose-Response Relationship, Drug
- Escherichia coli
- Kinetics
- Membrane Transport Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Proline
- Protein Structure, Secondary
- Sodium