Article
Alteration of Na(+)-coupled transport in site-directed mutants of the melibiose carrier of Escherichia coli.
Biochimica et biophysica acta - 25 Jul 1996
Franco P J, Wilson T H
Abstract excerpt
Asn-58 of the Escherichia coli melibiose carrier was replaced by Ala, Leu, Ser, and Gln. Trp-54 was replaced by Leu and a double mutant Leu-54/Ala-58 was constructed using site-directed mutagenesis. Cation/sugar cotransport and sugar-induced cation uptake were studied for each mutant. The change...
Topics
- Amino Acid Sequence
- Base Sequence
- Biological Transport
- Escherichia coli
- Hydrogen
- Lactose
- Melibiose
- Membrane Transport Proteins
- Methylgalactosides
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Sodium
- Structure-Activity Relationship
- Symporters
- Thiogalactosides