Evidence for a close association between helix IV and helix XI in the melibiose carrier of Escherichia coli.

doi:10.1016/s0005-2736(98)00132-1

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Article

Evidence for a close association between helix IV and helix XI in the melibiose carrier of Escherichia coli.

Biochimica et biophysica acta - 23 Sept 1998

Hastings Wilson T, Wilson D M

Abstract excerpt

The melibiose carrier of Escherichia coli is a cation-sugar cotransport protein. Asp124 in membrane-spanning helix IV of the carrier protein was replaced with Ser, Ile or Phe by site-directed mutagenesis of a plasmid containing the melB gene. Each of these mutants failed to show membrane transpor...

Topics

Base Sequence
Binding Sites
Biological Transport, Active
DNA Primers
Escherichia coli
Genes, Bacterial
Kinetics
Melibiose
Membrane Transport Proteins
Mutagenesis, Site-Directed
Phenotype
Protein Structure, Secondary
Symporters
Temperature

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