Article
Identification and characterization of functional residues in a Na+/H+ antiporter (NhaA) from Escherichia coli by random mutagenesis.
Journal of biochemistry - 1 Apr 1997
Noumi T, Inoue H, Sakurai T, Tsuchiya T, Kanazawa H
Abstract excerpt
Forty-one mutants were isolated by means of random PCR mutagenesis of the Escherichia coli Na+/H+ antiporter (nhaA), which could not support the growth of a nhaAnhaB mutant (HIT delta AB-) on plates containing 0.15 M LiCl (pH 7.5) or 0.65 M NaCl (pH 8.0). Most of the mutants were sensitive to bot...
Topics
- Amino Acid Sequence
- Binding Sites
- Escherichia coli
- Escherichia coli Proteins
- Hydrogen-Ion Concentration
- Lithium
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Protons
- Sodium
- Sodium-Hydrogen Exchangers
- Substrate Specificity
- Temperature