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Article

Alteration of sugar-induced conformational changes of the melibiose permease by mutating Arg141 in loop 4-5.

Biophysical journal - 17 Jun 2009

León Xavier, Leblanc Gérard, Padrós Esteve

Abstract excerpt

The melibiose permease (MelB) from Escherichia coli couples the uptake of melibiose to that of Na+, Li+, or H+. In this work, we applied attenuated total reflection Fourier transform infrared (ATR-FTIR) difference spectroscopy to obtain information about the structural changes involved in substrate interaction with the R141C mutant and with the wild-type MelB reacted with N-ethylmaleimide (NEM). These modified...

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Identifiers

PMID: 19527646
DOI: 10.1016/j.bpj.2009.03.025
PMCID: PMC2712053

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ATR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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