Article
Glutamate 85 is involved in the sodium/proton exchange activity of the Escherichia coli ChaA.
Bioscience, biotechnology, and biochemistry - 1 Jan 2010
Fukaya Fuminori, Tanaka Kimihiro, Waditee Rungaroon, Tanaka Yoshito, Nakamura Tatsunosuke, Takabe Teruhiro
Abstract excerpt
Hitherto, the roles of specific amino acid residues of ChaA, one of three Na(+)/H(+) antiporters in Escherichia coli, in exchange activity have not been reported. Here we examined the role of acidic amino acid residues, Glu-85 and Glu-325, on the hydrophobic transmembrane domains. It was found that ChaA is involved in salt tolerance at alkaline pH. Mutagenesis analyses revealed the importance of Glu-85, but not...
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