Article
Expression of cloned beta-tubulin genes of Haemonchus contortus in Escherichia coli: interaction of recombinant beta-tubulin with native tubulin and mebendazole.
Molecular and biochemical parasitology - 1 Dec 1993
Lubega G W, Geary T G, Klein R D, Prichard R K
Abstract excerpt
Two distinct beta-tubulin cDNA isotypes (beta 8-9 and beta 12-16) from Haemonchus contortus were expressed for the first time in Escherichia coli and characterised by their specific mebendazole (MBZ) binding and polymerization properties. Beta-tubulin was expressed without translational fusion to...
Topics
- Animals
- Blotting, Western
- Chickens
- Cloning, Molecular
- Drug Resistance
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression Regulation
- Haemonchus
- Mebendazole
- Mutation