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Article

Cercosporin-deficient mutants by plasmid tagging in the asexual fungus Cercospora nicotianae.

Molecular genetics and genomics : MGG - 1 Nov 2003

Chung K-R, Ehrenshaft M, Wetzel D K, Daub M E

Abstract excerpt

We have successfully adapted plasmid insertion and restriction enzyme-mediated integration (REMI) to produce cercosporin toxin-deficient mutants in the asexual phytopathogenic fungus Cercospora nicotianae. The use of pre-linearized plasmid or restriction enzymes in the transformation procedure significantly decreased the transformation frequency, but promoted a complicated and undefined mode of plasmid...

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PMID: 12937958
DOI: 10.1007/s00438-003-0902-7

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