Cloning yeast genes by complementation.

Abstract excerpt

This unit presents a generalized protocol and describes the principles involved in cloning yeast genes by complementation in yeast. The protocol is presented using a hypothetical mutation of yeast, the cdc101-1 mutation. This mutation was isolated as a cell cycle mutant and is both recessive and temperature-sensitive for growth: it can grow relatively normally at 30 degrees C but is unable to make a colony at 37...

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