Targeting of a multicomponent transcription apparatus into assembling vaccinia virus particles requires RAP94, an RNA polymerase-associated protein.

Abstract excerpt

When expression of the vaccinia virus gene encoding RAP94 (a protein that is associated with the viral multisubunit RNA polymerase and confers transcriptional specificity for early promoters) was repressed, the infectious virus yield was reduced by more than 99%. Nevertheless, intermediate- and late-stage viral gene expression and formation of ultrastructurally mature, membrane-enveloped virions occurred under...

Topics

• Base Sequence
• Cytoplasm
• DNA-Directed RNA Polymerases
• HeLa Cells
• Humans
• Models, Biological
• Molecular Sequence Data
• Mutation
• Promoter Regions, Genetic
• Transcription, Genetic
• Vaccinia virus