The crystal structure of affinity-matured human growth hormone at 2 A resolution.

doi:10.1006/jmbi.1994.1135

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Article

The crystal structure of affinity-matured human growth hormone at 2 A resolution.

Journal of molecular biology - 11 Feb 1994

Ultsch M H, Somers W, Kossiakoff A A, de Vos A M

Abstract excerpt

A variant of human growth hormone (hGH), in which 15 mutations were introduced with phage display mutagenesis to improve receptor binding affinity by 400-fold, yielded two related crystal forms diffracting to high resolution. The structure of this variant was determined in both crystal forms, one...

Topics

Amino Acid Sequence
Computer Graphics
Crystallography, X-Ray
Genetic Variation
Growth Hormone
Humans
Models, Molecular
Molecular Sequence Data
Mutagenesis
Protein Structure, Secondary
Recombinant Proteins

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