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Article

Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding.

The FEBS journal - 1 Aug 2005

Chen Hueih-Min, Chan Siu-Chiu, Leung King-Wong, Wu Jiun-Ming, Fang Huey-Jen, Tsong Tian Yow

Abstract excerpt

Staphylococcal nuclease is a single domain protein with 149 amino acids. It has no disulfide bonds, which makes it a simple model for the study of protein folding. In this study, 20 mutants of this protein were generated each with a single base substitution of glycine for negatively charged glutamic acid or aspartic acid. Using differential scanning microcalorimetry in thermal denaturation experiments, we...

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Source

Identifiers

PMID: 16045767
DOI: 10.1111/j.1742-4658.2005.04816.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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