Article
The processing of wild type and mutant forms of rat nuclear pre-tRNA(Lys) by the homologous RNase P.
Nucleic acids research - 25 Aug 1994
Paisley T E, Van Tuyle G C
Abstract excerpt
The 5' processing of rat pre-tRNA(Lys) and a series of mutant derivatives by rat cytosolic RNase P was examined. In standard, non-kinetic assays, mutant precursors synthesized in vitro with 5' leader sequences of 10, 17, 24, 25, and 46 nucleotides were processed to approximately equal levels and...
Topics
- Animals
- Base Composition
- Base Sequence
- Binding Sites
- Endoribonucleases
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- RNA Precursors
- RNA, Catalytic
- RNA, Transfer, Lys
- Rats
- Ribonuclease P
- Structure-Activity Relationship
- Substrate Specificity