Article
Effects of conserved D/T loop substitutions in the pre-tRNA substrate on tRNase Z catalysis.
RNA biology - 1 Jan 2000
Hopkinson Angela, Levinger Louis
Abstract excerpt
tRNAs are transcribed as precursors with a 5' end leader and a 3' end trailer. In the course of tRNA maturation, RNase P removes the 5' end leader and tRNase Z can endonucleolytically remove the 3' end trailer. A domain remote from the active site of tRNase Z recognizes and binds substrate, principally through contacts with the elbow (D/T loops) of the tRNA. To evaluate possible contacts, processing kinetics was...
Topics
- Bacillus subtilis
- Base Sequence
- Catalysis
- Conserved Sequence
- Endoribonucleases
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA Precursors
- RNA Processing, Post-Transcriptional
- RNA, Transfer, Arg
- RNA, Transfer, Val
- Substrate Specificity