Article
Plasmid transformation and replica filter plating of Acholeplasma laidlawii.
FEMS microbiology letters - 1 Oct 1990
Sundström T K, Wieslander A
Abstract excerpt
The restriction deficient mutant 8195 of Acholeplasma laidlawii strain JA1 was transformed by the promiscuous streptococcal plasmid vector pNZ18 at a frequency of 4 x 10(-4)/cfu. The plasmid was maintained without structural rearrangements but was lost in the absence of a selection pressure, i.e....
Topics
- Acholeplasma laidlawii
- Drug Resistance, Microbial
- Kanamycin Resistance
- Mutation
- Neomycin
- Phenotype
- Plasmids
- Restriction Mapping
- Transformation, Bacterial