Article
Plasmid transformation and replica filter plating of Acholeplasma laidlawii.
FEMS microbiology letters - 1 Oct 1990
Sundström T K, Wieslander A
Abstract excerpt
The restriction deficient mutant 8195 of Acholeplasma laidlawii strain JA1 was transformed by the promiscuous streptococcal plasmid vector pNZ18 at a frequency of 4 x 10(-4)/cfu. The plasmid was maintained without structural rearrangements but was lost in the absence of a selection pressure, i.e. kanamycin or neomycin. Transformed primary colonies were easily recognized due to a different colony morphology....
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