Article
An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency.
Gene - 1 Feb 1991
Gems D, Johnstone I L, Clutterbuck A J
Abstract excerpt
From an unstable Aspergillus nidulans colony, resulting from transformation with an A. nidulans gene bank, a plasmid was reisolated which transformed A. nidulans at a frequency of 20,000 transformants per 10(6) protoplasts at near saturation levels of transforming DNA. This represents a 250-fold enhancement of transformation efficiency over that found for typical integrative vectors such as pILJ16, the plasmid...
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