Article
An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency.
Gene - 1 Feb 1991
Gems D, Johnstone I L, Clutterbuck A J
Abstract excerpt
From an unstable Aspergillus nidulans colony, resulting from transformation with an A. nidulans gene bank, a plasmid was reisolated which transformed A. nidulans at a frequency of 20,000 transformants per 10(6) protoplasts at near saturation levels of transforming DNA. This represents a 250-fold...
Topics
- Aspergillus nidulans
- Blotting, Southern
- Centrifugation, Density Gradient
- Cloning, Molecular
- DNA Replication
- DNA, Fungal
- Genetic Linkage
- Genetic Markers
- Phenotype
- Plasmids
- Replicon
- Restriction Mapping
- Transformation, Genetic