Kex2-dependent invertase secretion as a tool to study the targeting of transmembrane proteins which are involved in ER-->Golgi transport in yeast.

doi:10.1002/j.1460-2075.1994.tb06679.x

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Article

Kex2-dependent invertase secretion as a tool to study the targeting of transmembrane proteins which are involved in ER-->Golgi transport in yeast.

The EMBO journal - 15 Aug 1994

Boehm J, Ulrich H D, Ossig R, Schmitt H D

Abstract excerpt

Mutants were isolated that are defective in the retention of a transmembrane protein in the early secretory compartments in yeast. A series of hybrid proteins was tested for their use in the selection of such mutants. Each of these hybrid proteins consisted of a type II transmembrane protein (Nin/Cout) and invertase (Suc2) as a reporter separated by a peptide linker containing a cleavage site for the Golgi...

Topics

Amino Acid Sequence
Base Sequence
Biological Transport
Endoplasmic Reticulum
Fungal Proteins
Genes, Fungal
Genes, Reporter
Glycoside Hydrolases
Glycosyltransferases
Golgi Apparatus
Guanine Nucleotide Exchange Factors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Membrane Glycoproteins

Membrane Proteins

Molecular Sequence Data

Mutation

Organelles

Proprotein Convertases

Recombinant Fusion Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 8070399
DOI: 10.1002/j.1460-2075.1994.tb06679.x
PMCID: PMC395280

Genes

RER1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8070399-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Kex2-dependent invertase secretion as a tool to study the targeting of transmembrane proteins which are involved in ER-->Golgi transport in yeast.PMID 8070399Read ↗

Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p.Semantic proximity Read ↗

Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteins.Semantic proximity Read ↗

Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth.Semantic proximity Read ↗

Active and specific recruitment of a soluble cargo protein for endoplasmic reticulum exit in the absence of functional COPII component Sec24p.Semantic proximity Read ↗

Introduction of Kex2 cleavage sites in fusion proteins for monitoring localization and transport in yeast secretory pathway.Semantic proximity Read ↗

Sec12p requires Rer1p for sorting to coatomer (COPI)-coated vesicles and retrieval to the ER.Semantic proximity Read ↗

Structural and functional dissection of a membrane glycoprotein required for vesicle budding from the endoplasmic reticulum.Semantic proximity Read ↗

Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum.Semantic proximity Read ↗

Immunoisolation of Kex2p-containing organelles from yeast demonstrates colocalisation of three processing proteinases to a single Golgi compartment.Semantic proximity Read ↗

Differential fates of invertase mutants in the yeast endoplasmic reticulum.Semantic proximity Read ↗

Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain.Semantic proximity Read ↗

Identification of potential regulatory elements for the transport of Emp24p.Semantic proximity Read ↗

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