Generalized transposon shuttle mutagenesis in Neisseria gonorrhoeae: a method for isolating epithelial cell invasion-defective mutants.

doi:10.1111/j.1365-2958.1994.tb01068.x

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Article

Generalized transposon shuttle mutagenesis in Neisseria gonorrhoeae: a method for isolating epithelial cell invasion-defective mutants.

Molecular microbiology - 1 Jun 1994

Kahrs A F, Bihlmaier A, Facius D, Meyer T F

Abstract excerpt

One requirement for the invasion of, and tight adherence to, human epithelial cells by Neisseria gonorrhoeae is the synthesis of distinct opacity (Opa) outer membrane proteins, encoded by a family of phase-variable chromosomal genes. However, cloning and surface expression of invasion-promoting O...

Topics

Biological Transport
Cells, Cultured
DNA Transposable Elements
Epithelium
Escherichia coli
Genomic Library
Humans
Mutagenesis, Insertional
Mutation
Neisseria gonorrhoeae
Virulence

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