Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

doi:10.1128/jb.172.1.40-46.1990

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Article

Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

Journal of bacteriology - 1 Jan 1990

Seifert H S, Ajioka R S, Paruchuri D, Heffron F, So M

Abstract excerpt

The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express high...

Topics

Bacterial Outer Membrane Proteins
Chloramphenicol O-Acetyltransferase
DNA Transposable Elements
DNA, Bacterial
Drug Resistance, Microbial
Fimbriae Proteins
Mutation
Neisseria gonorrhoeae
Transformation, Genetic

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