Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Heterologous expression and characterization of wild-type human cytochrome P450 1A2 without conventional N-terminal modification in Escherichia coli.

Protein expression and purification - 1 Feb 2008

Kim Dong-Hyun, Kim Keon-Hee, Isin Emre M, Guengerich F Peter, Chae Ho Zoon, Ahn Taeho, Yun Chul-Ho

Abstract excerpt

In this study, wild-type human CYP1A2 without the conventional N-terminal modification (second codon GCT) or the truncation of the N-terminal hydrophobic region was functionally expressed in Escherichia coli. Its enzymatic properties were compared with N-terminally modified CYP1A2. Although modified CYP1A2 is almost all high-spin, some wild-type CYP1A2 shifted to low-spin. Spectral binding titrations with several...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cytochrome P-450 CYP1A2
Share to Deuterium

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Enzyme Inhibitors

Share to Hydrogen Peroxide

Share to Hydroxylation

Share to Oxidation-Reduction

Share to Spectrophotometry

Source

Identifiers

PMID: 18032064
DOI: 10.1016/j.pep.2007.10.010

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Heterologous expression and characterization of wild-type human cytochrome P450 1A2 without conventional N-terminal modification in Escherichia coli.PMID 18032064Read ↗

Expression of modified human cytochrome P450 1A1 in Escherichia coli: effects of 5' substitution, stabilization, purification, spectral characterization, and catalytic properties.Semantic proximity Read ↗

Significant increase in phenacetin oxidation on L382V substitution in human cytochrome P450 1A2.Semantic proximity Read ↗

Terfenadine metabolism of human cytochrome P450 2J2 containing genetic variations (G312R, P351L and P115L).Semantic proximity Read ↗

Functional characterization of four allelic variants of human cytochrome P450 1A2.Semantic proximity Read ↗

Expression of modified human cytochrome P450 2E1 in Escherichia coli, purification, and spectral and catalytic properties.Semantic proximity Read ↗

Impact of single nucleotide polymorphisms (R132Q and W120R) on the binding affinity and metabolic activity of CYP2C19 toward some therapeutically important substrates.Semantic proximity Read ↗

The role of cytochrome 2B1 substrate recognition site residues 115, 294, 297, 298, and 362 in the oxidation of steroids and 7-alkoxycoumarins.Semantic proximity Read ↗

Biochemical analysis of recombinant CYP4A11 allelic variant enzymes: W126R, K276T and S353G.Semantic proximity Read ↗

Site-Directed Mutagenesis of Cytochrome P450 2D6 and 2C19 Enzymes: Expression and Spectral Characterization of Naturally Occurring Allelic Variants.Semantic proximity Read ↗

Enhancement of 7-methoxyresorufin O-demethylation activity of human cytochrome P450 1A2 by molecular breeding.Semantic proximity Read ↗

Stoichiometry of 7-ethoxycoumarin metabolism by cytochrome P450 2B1 wild-type and five active-site mutants.Semantic proximity Read ↗

A novel methodology for enhanced and consistent heterologous expression of unmodified human cytochrome P450 1B1 (CYP1B1).Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.