Article
The anticoagulant thrombin mutant W215A/E217A has a collapsed primary specificity pocket.
The Journal of biological chemistry - 17 Sept 2004
Pineda Agustin O, Chen Zhi-Wei, Caccia Sonia, Cantwell Angelene M, Savvides Savvas N, Waksman Gabriel, Mathews F Scott, Di Cera Enrico
Abstract excerpt
The thrombin mutant W215A/E217A features a drastically impaired catalytic activity toward chromogenic and natural substrates but efficiently activates the anticoagulant protein C in the presence of thrombomodulin. As the remarkable anticoagulant properties of this mutant continue to be unraveled in preclinical studies, we solved the x-ray crystal structures of its free form and its complex with the active site...
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