Article
Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III.
Acta crystallographica. Section D, Biological crystallography - 1 Sept 2012
Mikami Bunzo, Ban Mizuho, Suzuki Sachiko, Yoon Hye-Jin, Miyake Osamu, Yamasaki Masayuki, Ogura Kohei, Maruyama Yukie, Hashimoto Wataru, Murata Kousaku
Abstract excerpt
The structures of two mutants (H192A and Y246F) of a mannuronate-specific alginate lyase, A1-III, from Sphingomonas species A1 complexed with a tetrasaccharide substrate [4-deoxy-L-erythro-hex-4-ene-pyranosyluronate-(mannuronate)(2)-mannuronic acid] were determined by X-ray crystallography at around 2.2 Å resolution together with the apo form of the H192A mutant. The final models of the complex forms, which...
Topics
- Amino Acid Sequence
- Apoenzymes
- Biocatalysis
- Holoenzymes
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Polysaccharide-Lyases
- Protein Structure, Tertiary
- Sequence Alignment
- Sphingomonas