Article
Human immunoglobulin transgenes undergo rearrangement, somatic mutation and class switching in mice that lack endogenous IgM.
International immunology - 1 Apr 1994
Taylor L D, Carmack C E, Huszar D, Higgins K M, Mashayekh R, Sequar G, Schramm S R, Kuo C C, O'Donnell S L, Kay R M
Abstract excerpt
We have generated transgenic mice that contain human-sequence Ig miniloci and, because they are also homozygous for a targeted disruption of their endogenous heavy chain genes, must rely on the transgene sequences for B cell receptor expression. Although the human transgenes contain only a fracti...
Topics
- Animals
- B-Lymphocytes
- Base Sequence
- Blotting, Southern
- Cell Differentiation
- Flow Cytometry
- Gene Rearrangement, B-Lymphocyte, Heavy Chain
- Genes, Immunoglobulin
- Humans
- Immunization
- Immunoglobulin Class Switching
- Immunoglobulin Heavy Chains
- Lymphocyte Transfusion