Article
Mice with megabase humanization of their immunoglobulin genes generate antibodies as efficiently as normal mice.
Proceedings of the National Academy of Sciences of the United States of America - 8 Apr 2014
Murphy Andrew J, Macdonald Lynn E, Stevens Sean, Karow Margaret, Dore Anthony T, Pobursky Kevin, Huang Tammy T, Poueymirou William T, Esau Lakeisha, Meola Melissa, Mikulka Warren, Krueger Pamela, Fairhurst Jeanette, Valenzuela David M, Papadopoulos Nicholas, Yancopoulos George D
Abstract excerpt
Mice genetically engineered to be humanized for their Ig genes allow for human antibody responses within a mouse background (HumAb mice), providing a valuable platform for the generation of fully human therapeutic antibodies. Unfortunately, existing HumAb mice do not have fully functional immune systems, perhaps because of the manner in which their genetic humanization was carried out. Heretofore, HumAb mice have...
Topics
- Alleles
- Animals
- Antibody Formation
- B-Lymphocytes
- Flow Cytometry
- Genes, Immunoglobulin
- Humans
- Mice
- Mutation