Article
Antigen-specific human antibodies from mice comprising four distinct genetic modifications.
Nature - 28 Apr 1994
Lonberg N, Taylor L D, Harding F A, Trounstine M, Higgins K M, Schramm S R, Kuo C C, Mashayekh R, Wymore K, McCabe J G
Abstract excerpt
Human sequence monoclonal antibodies, which in theory combine high specificity with low immunogenicity, represent a class of potential therapeutic agents. But nearly 20 years after Köhler and Milstein first developed methods for obtaining mouse antibodies, no comparable technology exists for reli...
Topics
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- B-Lymphocytes
- Base Sequence
- Bone Marrow Cells
- Female
- Gene Rearrangement, B-Lymphocyte
- Humans
- Hybridomas
- Immunoglobulin E
- Immunoglobulin Heavy Chains
- Immunoglobulin Light Chains
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides