Article
Functional analysis of the cysteine residues of activin A.
Molecular endocrinology (Baltimore, Md.) - 1 Mar 1994
Mason A J
Abstract excerpt
Site-directed mutagenesis and mammalian cell expression was used to analyze the function of each of the 13 cysteine residues in the human activin A beta-subunit precursor. Substitution of the four cysteine residues in the proregion with alanine residues did not affect the function of the proregion in facilitating the dimerization and secretion of activin A homodimers. A series of activin mutants were constructed...
Topics
- Activins
- Alanine
- Amino Acid Sequence
- Cell Line
- Cysteine
- Escherichia coli
- Humans
- Inhibins
- Kidney
- Molecular Sequence Data
- Mutation
- Protein Precursors