Article
Heterologous expression of the human D2S dopamine receptor in protease-deficient Saccharomyces cerevisiae strains.
European journal of biochemistry - 1 Dec 1994
Sander P, Grünewald S, Bach M, Haase W, Reiländer H, Michel H
Abstract excerpt
The cDNA for the human D2S dopamine receptor has been functionally expressed in the unicellular yeast Saccharomyces cerevisiae. The original D2S gene and an elongated D2S gene with an N-terminal fusion to the first 24 amino acids of the STE2 gene from S. cerevisiae were introduced into the episom...
Topics
- Base Sequence
- Binding, Competitive
- Blotting, Northern
- Cytoplasm
- Endopeptidases
- Gene Expression
- Gene Transfer Techniques
- Humans
- Immunoblotting
- Immunohistochemistry
- Molecular Sequence Data
- Mutation
- Plasmids
- Receptors, Dopamine D2
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Spiperone
- Vacuoles