Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptor.

Nucleic acids research - 1 Apr 2008

Dirnberger Dietmar, Messerschmid Monika, Baumeister Ralf

Abstract excerpt

The yeast split-ubiquitin system has previously been shown to be suitable to detect protein interactions of membrane proteins and of transcription factors in vivo. Therefore, this technology complements the classical split-transcription factor based yeast two-hybrid system (Y2H). Success or failu...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Frizzled Receptors
Share to Fungal Proteins
Share to Gene Library

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Orotic Acid

Share to Protein Interaction Mapping

Share to Recombinant Fusion Proteins

Share to Ubiquitin

Share to Yeasts

Source

Open in PubMed Open full text

Identifiers

PMID: 18319286
DOI: 10.1093/nar/gkm1163
PMCID: PMC2330230

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-18319286-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptor.PMID 18319286Read ↗

Rapid, Facile Detection of Heterodimer Partners for Target Human G-Protein-Coupled Receptors Using a Modified Split-Ubiquitin Membrane Yeast Two-Hybrid System.Semantic proximity Read ↗

Yeast one-hybrid screens for detection of transcription factor DNA interactions.Semantic proximity Read ↗

Identification of Suitable Target/E3 Ligase Pairs for PROTAC Development using a Rapamycin-induced Proximity Assay (RiPA)Semantic proximity Read ↗

Identification of She3 as an SCF(Grr1) substrate in budding yeast.Semantic proximity Read ↗

A bacterial/yeast merged two-hybrid system: protocol for yeast screening with single or parallel baits.Semantic proximity Read ↗

One- plus two-hybrid system for the efficient selection of missense mutant alleles defective in protein-protein interactions.Semantic proximity Read ↗

Application of the split-ubiquitin membrane yeast two-hybrid system to investigate membrane protein interactions.Semantic proximity Read ↗

A highly efficient and sensitive screening method for trans-activation activity of estrogen receptors.Semantic proximity Read ↗

A ubiquitin-protein ligase (E3) mutation of Saccharomyces cerevisiae suppressed by co-overexpression of two ubiquitin-specific processing proteases.Semantic proximity Read ↗

A two-step two-hybrid system to identify functionally significant protein-protein interactions.Semantic proximity Read ↗

Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification.Semantic proximity Read ↗

A viable ubiquitin-activating enzyme mutant for evaluating ubiquitin system function in Saccharomyces cerevisiae.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.