Article
Surface labeling of key residues during assembly of the transmembrane pore formed by staphylococcal alpha-hemolysin.
FEBS letters - 12 Dec 1994
Krishnasastry M, Walker B, Braha O, Bayley H
Abstract excerpt
Structural changes in staphylococcal alpha-hemolysin (alpha HL) that occur during oligomerization and pore formation on membranes have been examined by using a simple gel-shift assay to determine the rate of modification of key single-cysteine mutants with the hydrophilic sulfhydryl reagent, 4-ac...
Topics
- Animals
- Bacterial Toxins
- Cysteine
- Erythrocytes
- Hemolysin Proteins
- Histidine
- Mutation
- Rabbits
- Staphylococcus aureus
- Stilbenes