Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activity.

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Article

Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activity.

The Journal of biological chemistry - 25 May 1992

Walker B, Krishnasastry M, Zorn L, Kasianowicz J, Bayley H

Abstract excerpt

The alpha-hemolysin gene from Staphylococcus aureus, excluding the 5' region encoding the hydrophobic leader sequence, was amplified from genomic DNA. The identity of the disputed C terminus has been confirmed and revisions made to the internal sequence. The hemolysin is expressed at high levels...

Topics

Animals
Autoradiography
Bacterial Toxins
Base Sequence
Chromosome Deletion
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Erythrocytes
Escherichia coli
Genes, Bacterial
Hemolysin Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hemolysis

Hydrogen-Ion Concentration

Kinetics

Lipid Bilayers

Molecular Sequence Data

Mutation

Plasmids

Source

Open in PubMed

Identifiers

PMID: 1587866

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1587866-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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