Identification and characterization of a temperature-sensitive mutant of human immunodeficiency virus type 1 by alanine scanning mutagenesis of the integrase gene.

doi:10.1128/JVI.69.1.597-601.1995

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Article

Identification and characterization of a temperature-sensitive mutant of human immunodeficiency virus type 1 by alanine scanning mutagenesis of the integrase gene.

Journal of virology - 1 Jan 1995

Wiskerchen M, Muesing M A

Abstract excerpt

We have created a temperature-sensitive (ts) mutant of human immunodeficiency virus type 1, using the technique of charge-cluster-to-alanine scanning mutagenesis to introduce specific changes into the integrase coding region. In the ts mutant virus, the lysine at amino acid 136 and the glutamic a...

Topics

Alanine
Cell Line
DNA Nucleotidyltransferases
HIV-1
Humans
Integrases
Mutagenesis
Mutation
Phenotype
Temperature
Virus Integration

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