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Article

Identification and characterization of a temperature-sensitive mutant of human immunodeficiency virus type 1 by alanine scanning mutagenesis of the integrase gene.

Journal of virology - 1 Jan 1995

Wiskerchen M, Muesing M A

Abstract excerpt

We have created a temperature-sensitive (ts) mutant of human immunodeficiency virus type 1, using the technique of charge-cluster-to-alanine scanning mutagenesis to introduce specific changes into the integrase coding region. In the ts mutant virus, the lysine at amino acid 136 and the glutamic acid at amino acid 138 of integrase have been replaced with alanines (K136A/E138A). When K136A/E138A is synthesized at...

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Identifiers

PMID: 7983762
DOI: 10.1128/JVI.69.1.597-601.1995
PMCID: PMC188617

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