Mutations in the Caenorhabditis elegans beta-tubulin gene mec-7: effects on microtubule assembly and stability and on tubulin autoregulation.

Abstract excerpt

We have sequenced 45 mutations in mec-7, a beta-tubulin gene required for the production of 15-protofilament microtubules in the nematode Caenorhabditis elegans, and have correlated sequence alterations with mutant phenotypes. The expression patterns of most alleles have also been determined by in situ hybridization and immunocytochemistry. Most (12/16) complete loss-of-function alleles, which are recessive,...

Topics

• Alleles
• Animals
• Caenorhabditis elegans
• Fluorescent Antibody Technique
• Genes, Helminth
• In Situ Hybridization
• Mechanoreceptors
• Microtubules
• Mutagens
• Mutation
• Phenotype