Thymine starvation-induced structural changes in Escherichia coli DNA. Detection by pulsed field gel electrophoresis and evidence for involvement of homologous recombination.

Abstract excerpt

Effect of thymine starvation on Escherichia coli DNA was investigated by using pulsed field gel electrophoresis combined with cell lysis in agarose gel. Post-lysis treatment with restriction enzymes generating relatively large fragments (NheI, SpeI or XbaI) revealed peculiar electrophoretic profiles specific for thymine-starved cells. Thus, a substantial portion of the DNA remained in the origin of...

Topics

• Biotransformation
• Cell Survival
• Cells, Cultured
• DNA Replication
• DNA, Bacterial
• Electrophoresis, Gel, Pulsed-Field
• Escherichia coli
• Microscopy, Electron
• Mutation
• Nucleic Acid Hybridization