Molecular cloning of the wild-type and mutant thyA gene from Shigella flexneri Y.

doi:10.1111/j.1348-0421.1994.tb01782.x

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Article

Molecular cloning of the wild-type and mutant thyA gene from Shigella flexneri Y.

Microbiology and immunology - 1 Jan 1994

Nur-E-Kamal M S, al Mamun A A, Ahmed Z U

Abstract excerpt

The thyA gene which codes for thymidylate synthase has been cloned and sequenced from the wild-type Shigella flexneri Y strain SH4 and a thyA mutant TSF21 after amplifying the gene by polymerase chain reaction (PCR). The nucleotide sequence revealed 98% homology to the E. coli K-12 thyA gene. The...

Topics

Base Sequence
Cloning, Molecular
Escherichia coli
Genes, Bacterial
Molecular Sequence Data
Mutation
Phenotype
Polymerase Chain Reaction
Sequence Homology, Nucleic Acid
Shigella flexneri
Thymidylate Synthase

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