Heat-stable and heat-labile thymidylate synthases B of Bacillus subtilis: comparison of the nucleotide and amino acid sequences.

doi:10.1007/BF00281594

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Article

Heat-stable and heat-labile thymidylate synthases B of Bacillus subtilis: comparison of the nucleotide and amino acid sequences.

Molecular & general genetics : MGG - 1 May 1993

Montorsi M, Lorenzetti R

Abstract excerpt

Bacillus subtilis 168 is known to possess two thymidylate synthase (TSase; EC 2.1.1.45) genes: thyA and thyB. thyB encodes a thermosensitive TSase (inactivated at 46 degrees C) which, in wild-type cells, accounts for only 5-8% of the total cellular TSase activity. In order to investigate the ther...

Topics

Amino Acid Sequence
Bacillus subtilis
Base Sequence
Cloning, Molecular
DNA, Bacterial
Enzyme Stability
Hot Temperature
Molecular Sequence Data
Mutation
Phenotype
Restriction Mapping
Thymidylate Synthase

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