Article
A transcription terminator in the thymidylate synthase (thyA) structural gene of Escherichia coli and construction of a viable thyA::Kmr deletion.
Journal of bacteriology - 1 Feb 1991
Bell-Pedersen D, Galloway Salvo J L, Belfort M
Abstract excerpt
A transcription terminator has been identified within the coding sequence of the Escherichia coli thyA gene. Fusion of a relevant segment of the thyA structural gene to galK sequences showed that the terminator functions in vivo. Primer extension and Northern hybridization (RNA blot) analysis of...
Topics
- Alleles
- Base Sequence
- Blotting, Northern
- Chromosome Deletion
- Chromosomes, Bacterial
- Escherichia coli
- Exons
- Genes, Bacterial
- Molecular Sequence Data
- Nucleic Acid Conformation
- Restriction Mapping
- Terminator Regions, Genetic
- Thymidylate Synthase
- Transcription, Genetic
- Transduction, Genetic