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Article

Synergistic insertion of two hydrophobic regions drives Sec-independent membrane protein assembly.

The Journal of biological chemistry - 28 Oct 1994

Cao G, Cheng S, Whitley P, von Heijne G, Kuhn A, Dalbey R E

Abstract excerpt

We have studied the membrane insertion of two proteins from the inner membrane of Escherichia coli, both with two transmembrane segments connected by a short periplasmic loop: the M13 procoat protein and a mutant "inverted" leader peptidase. Neither molecule depends on the Sec machinery for insertion. We show that the introduction of a charged residue in the second transmembrane segment completely blocks...

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PMID: 7929429

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Click a neighbor to travelStep 1 · 12 closest

Synergistic insertion of two hydrophobic regions drives Sec-independent membrane protein assembly.PMID 7929429Read ↗

Indecisive M13 procoat protein mutants bind to SecA but do not activate the translocation ATPase.Semantic proximity Read ↗

M13 procoat protein insertion into YidC and SecYEG proteoliposomes and liposomes.Semantic proximity Read ↗

Efficient translocation of positively charged residues of M13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion.Semantic proximity Read ↗

Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction.Semantic proximity Read ↗

Polarity and charge of the periplasmic loop determine the YidC and sec translocase requirement for the M13 procoat lep protein.Semantic proximity Read ↗

Features of membrane protein sequence direct post-translational insertion.Semantic proximity Read ↗

Membrane protein sequence features direct post-translational insertionSemantic proximity Read ↗

Sec dependent and sec independent assembly of E. coli inner membrane proteins: the topological rules depend on chain length.Semantic proximity Read ↗

The translocation of negatively charged residues across the membrane is driven by the electrochemical potential: evidence for an electrophoresis-like membrane transfer mechanism.Semantic proximity Read ↗

The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins.Semantic proximity Read ↗

SecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particle.Semantic proximity Read ↗

Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC.Semantic proximity Read ↗

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