Article
Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction.
The EMBO journal - 1 Sept 1990
Gallusser A, Kuhn A
Abstract excerpt
Bacteriophage M13 procoat protein is synthesized on free polysomes prior to its assembly into the inner membrane of Escherichia coli. As an initial step of the membrane insertion pathway, the precursor protein interacts with the cytoplasmic face of the inner membrane. We have used oligonucleotide-directed mutagenesis to study the regions of the procoat protein involved in membrane binding. We find that there is...
Topics
- Amino Acid Sequence
- Base Sequence
- Capsid
- Cell Membrane
- Chromosome Deletion
- Coliphages
- Electrochemistry
- Escherichia coli
- Liposomes
- Molecular Sequence Data
- Mutation