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Article

Computation-Guided Rational Design of Cysteine-Less Protein Variants in Engineered hCGL.

Journal of agricultural and food chemistry - 1 May 2024

Zhang Qian, Fan Shuai, Tang Mengjia, Wang Chenyu, Li Xiaoxiao, Jin Yuanyuan, Yang Zhaoyong

Abstract excerpt

The engineered human cystathionine-γ-lyase (hCGL) resulting in enhanced activity toward both cysteine and cystine unveils a potential robust antitumor activity. However, the presence of cysteine residues has the potential to induce oligomerization or incorrect disulfide bonding, which may decrease the bioavailability of biopharmaceuticals. Through a meticulous design process targeting the cysteine residues within...

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Identifiers

PMID: 38651303
DOI: 10.1021/acs.jafc.3c06821

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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