Hairpin loop mutations of chicken cystatin have different effects on the inhibition of cathepsin B, cathepsin L and papain.

doi:10.1016/0014-5793(95)00175-9

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Article

Hairpin loop mutations of chicken cystatin have different effects on the inhibition of cathepsin B, cathepsin L and papain.

FEBS letters - 20 Mar 1995

Auerswald E A, Nägler D K, Assfalg-Machleidt I, Stubbs M T, Machleidt W, Fritz H

Abstract excerpt

Five recombinant hairpin loop variants of chicken cystatin (delta V55, delta V55-S56, delta P103-L105, delta I102-Q107, loop2-KD2) were constructed by cassette mutagenesis, expressed in E. coli, purified to homogeneity, characterized by protein-chemical means and by their inhibitory properties. The variant forms, modified in two of the three postulated cysteine proteinase binding regions, were inhibitorily...

Topics

Amino Acid Sequence
Animals
Base Sequence
Cathepsin B
Cathepsin L
Cathepsins
Chickens
Cloning, Molecular
Cystatins
Cysteine Endopeptidases
Endopeptidases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genetic Variation

Kinetics

Molecular Sequence Data

Mutagenesis, Insertional

Oligodeoxyribonucleotides

Papain

Protein Structure, Secondary

Source

Open in PubMed

Identifiers

PMID: 7698319
DOI: 10.1016/0014-5793(95)00175-9

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7698319-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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