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Article

Melting of a DNA helix terminus within the active site of a DNA polymerase.

Biochemistry - 4 Oct 1994

Hochstrasser R A, Carver T E, Sowers L C, Millar D P

Abstract excerpt

Accurate synthesis of DNA by polymerase is due in part to the selective removal of misincorporated nucleotides by a 3'-5' exonuclease activity (proofreading). Proofreading by an exonuclease domain containing a single-stranded DNA binding site may involve local melting of a duplex DNA substrate. Here we use time-resolved fluorescence spectroscopy to analyze the local melting of a DNA duplex terminus induced by the...

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PMID: 7918416
DOI: 10.1021/bi00205a036

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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