Melting of a DNA helix terminus within the active site of a DNA polymerase.

doi:10.1021/bi00205a036

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Article

Melting of a DNA helix terminus within the active site of a DNA polymerase.

Biochemistry - 4 Oct 1994

Hochstrasser R A, Carver T E, Sowers L C, Millar D P

Abstract excerpt

Accurate synthesis of DNA by polymerase is due in part to the selective removal of misincorporated nucleotides by a 3'-5' exonuclease activity (proofreading). Proofreading by an exonuclease domain containing a single-stranded DNA binding site may involve local melting of a duplex DNA substrate. H...

Topics

2-Aminopurine
Base Composition
Base Sequence
DNA
DNA Polymerase I
DNA Primers
Escherichia coli
Half-Life
Molecular Sequence Data
Mutation
Nucleic Acid Denaturation
Spectrometry, Fluorescence

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