Article
Dynamic spin label study of the barstar-barnase complex.
Biochemistry. Biokhimiia - 1 Sept 2007
Timofeev V P, Balandin T G, Tkachev Ya V, Novikov V V, Lapuk V A, Deev S M
Abstract excerpt
The dynamic spin label method was used to study protein-protein interactions in the model complex of the enzyme barnase (Bn) with its inhibitor barstar. The C40A mutant of barstar (Bs) containing a single cysteine residue was modified with two different spin labels varying in length and structure of a flexible linker. Each spin label was selectively bound to the Cys82 residue, located near the Bn-Bs contact site....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.