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[Construction and in vitro assay of the sortase BCD geneknock-out mutant of Streptococcus suis 2].

Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Mar 2011

Chen Hongna, Liao Hui, Wang Changjun, Pan Xiuzhen, Tang Jiaqi

Abstract excerpt

OBJECTIVE: Streptococcus suis 2 is an emerging zoonotic pathogen responsible for a wide range of life-threatening diseases in pigs and humans. In this study, we investigated the functionality of one of Streptococcus suis 2 sortases, known as the srtBCD. METHODS: To obtain the isogenic mutant srtBCD, the competent cells of 05ZYH33 were subjected to electrotrans formation with recombinant plasmid based on the...

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PMID: 21604553

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