Article
Limited and defined truncation at the C terminus enhances receptor binding and degranulation activity of the neutrophil-activating peptide 2 (NAP-2). Comparison of native and recombinant NAP-2 variants.
The Journal of biological chemistry - 17 Mar 1995
Ehlert J E, Petersen F, Kubbutat M H, Gerdes J, Flad H D, Brandt E
Abstract excerpt
We have previously described a C-terminally truncated variant of the chemokine neutrophil-activating peptide 2 (NAP-2) that exhibited higher neutrophil-stimulating capacity than the full-size polypeptide. To investigate the impact of the NAP-2 C terminus on biological activity and receptor binding, we have now purified the novel molecule to homogeneity. Furthermore, we have cloned, expressed in Escherichia coli,...
Topics
- Amino Acid Sequence
- Base Sequence
- Blood Platelets
- Blotting, Western
- Cloning, Molecular
- Connective Tissue
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genetic Variation