Article
Coculture of human articular chondrocytes with peripheral blood mononuclear cells as a model to study cytokine-mediated interactions between inflammatory cells and target cells in the rheumatoid joint.
In vitro cellular & developmental biology. Animal - 1 Nov 1994
Malfait A M, Verbruggen G, Almqvist K F, Broddelez C, Veys E M
Abstract excerpt
A model for the coculture of chondrocytes in gelified agarose with mononuclear cells was developed to serve as an in vitro equivalent for cytokine-mediated events at the cartilage-synovial pannus junction in destructive arthropathies. Chondrocytes cultured in agarose keep their phenotypic stability. They release cartilage-specific aggrecans into the surrounding artificial matrix. When activated with...
Topics
- Adult
- Aggrecans
- Arthritis, Rheumatoid
- Cartilage, Articular
- Cell Communication
- Cells, Cultured
- Cytokines
- Extracellular Matrix Proteins
- Female
- Humans