Enzymatic isolation of chondrocytes from immature rabbit articular cartilage and maintenance of phenotypic expression in culture.

Abstract excerpt

The studies included here identify factors affecting cartilage digestion by crude bacterial collagenase (cCGN) and describe a cartilage digestion medium that maximizes both tissue digestion rate and viable cell yield. The basal digestion medium contained 100 mM NaCl, 3 mM K2HPO4, 1 mM CaCl2, 1 mM MgSO4, 10 mM NaHCO3, 60 mM sorbitol, 5 mg/ml of dextrose, 1 mg/ml of albumin, and 2 mg/ml of cCGN in 25 mM HEPES at pH...

Topics

• Animals
• Cartilage, Articular
• Cell Separation
• Cells, Cultured
• Collagen
• Male
• Microbial Collagenase
• Phenotype
• Proteoglycans
• Rabbits
• Sulfur Radioisotopes