Article
Expression and purification of a truncated recombinant streptococcal protein G.
The Biochemical journal - 1 Apr 1990
Goward C R, Murphy J P, Atkinson T, Barstow D A
Abstract excerpt
The gene for Protein G from Streptococcus strain G148 was cloned and expressed in Escherichia coli. The regions on the gene corresponding to the albumin-binding domains and the Fab-binding region were then deleted by site-directed mutagenesis. The translation of regions corresponding to the cell-wall- and membrane-binding domains was prevented by introduction of stop codons upstream of these domains. This...
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