Article
Expression and purification of a truncated recombinant streptococcal protein G.
The Biochemical journal - 1 Apr 1990
Goward C R, Murphy J P, Atkinson T, Barstow D A
Abstract excerpt
The gene for Protein G from Streptococcus strain G148 was cloned and expressed in Escherichia coli. The regions on the gene corresponding to the albumin-binding domains and the Fab-binding region were then deleted by site-directed mutagenesis. The translation of regions corresponding to the cell-...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Blotting, Western
- Cloning, Molecular
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- Immunoglobulin Fab Fragments
- Immunoglobulin Fc Fragments
- Immunoglobulin G
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Restriction Mapping
- Serum Albumin