HLA-DPA1 typing by PCR amplification with sequence-specific primers (PCR-SSP) and distribution of DPA1 alleles in Caucasian, African and Oriental populations.

Abstract excerpt

In the present study PCR primers were designed for detecting all known DPA1 variability, i.e., the presently recognized six DPA1 alleles 0103 to 0401, and also for separation of the four DPA1*02 alleles, by PCR amplification with sequence-specific primers (PCR-SSP). For each sample seven different PCR reactions were performed which allowed the identification of all DPA1 alleles and the resolution of all DPA1...

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